LeProlif Sphere Microcarriers
The spherical carriers are cultivated in benchtop bioreactors
Application of cultivating Vero cells and performing bead-to-bead expansion
● Based on cross-linked dextran
● Surface area approximately 4400cm2/g
● Special surface modification process enhances cell adhesion rate and uniformity, supporting high-density perfusion culture
● Can be used for suspension culture in glass bioreactors/single-use bioreactors, facilitating scale-up
● Cells can be harvested by releasing them off the microsphere surface
|Crosslinked- dextran matrix, modified with positively DEAE* group
|140-220μm in saline
|Autoclaved sterilization（121 ℃,30min）
This study conducted sterile inoculation of Vero cells on microcarriers in 2L and 10L glass vessels, controlling the process through a benchtop bioreactor. In this test, the microcarrier concentration was low, and regular culture methods were employed without the need for additional feeding or media changes, achieving cell proliferation and expansion. The cultured Vero cells attained a high density; with a microcarrier concentration of 3g/L and an initial seeding density of 1.2×105 cells/mL, by the fourth day, the cell density reached around 1.4×106 cells/mL, maintaining a viability of over 90%. Cells were harvested by enzymatically digesting them off the spheres and transferred to new or existing spherical carriers, enabling an expanded culture by transferring cells from one sphere to another. Additionally, increasing the carrier concentration can boost cell density. This confirms the significant potential of using LeProlif™ Sphere to cultivate Vero cells for vaccine production.
Materials & Equipement
In the 2L glass jar benchtop bioreactor, we conducted tests on the growth of Vero cells on spherical carriers to understand the proliferation characteristics of Vero cells within the benchtop bioreactor. Subsequently, we further amplified this process to the 10L glass bioreactor through bead-to-bead expansion, providing relevant references for the application of Vero cells in virus preparation.
With a carrier concentration of 3g/L and an initial cell seeding density of 1.2E5/mL, after 4 days of cultivation, scaling up from 1.5L to 4L through bead-to-bead expansion, and further scaling up to 10L, the cell density increased approximately 14-fold while maintaining a viability consistently above 90.0%. Across different scales of enlargement within glass jars, there was a high level of consistency observed, demonstrating favorable bead-to-bead effects, as depicted in Figures.
Datasheet-LeProlif Sphere Microcarriers
Manual-LeProlif Sphere Microcarrier