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LeProlif Sphere Microcarriers

Short Description:

Vero cells are a type of adherent cells widely used as a platform for producing virus vaccines. In a benchtop bioreactor, Vero cells typically grow on the surface of spherical microcarriers.

 

LeProlif Sphere spherical microcarriers are used to cultivate adherent cells at high yields. They provide a suitable surface for the growth of animal cells and can be used in a suspension cultivation system. They can also increase cell yields in single-layer cell culture vessels (such as culture plates, dishes, and bottles) and perfusion chambers.


Basic information

Parameters

Case Study

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LeProlif Sphere

The spherical carriers are cultivated in benchtop bioreactors
Application of cultivating Vero cells and performing bead-to-bead expansion

● Based on cross-linked dextran

● Surface area approximately 4400cm2/g

● Special surface modification process enhances cell adhesion rate and uniformity, supporting high-density perfusion culture

● Can be used for suspension culture in glass bioreactors/single-use bioreactors, facilitating scale-up

● Cells can be harvested by releasing them off the microsphere surface

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  • Items Parameters
    Materials Crosslinked- dextran matrix, modified with positively DEAE* group
    Size 140-220μm in saline
    Surface Area ~4400 cm²/g
    Sterilization Method Autoclaved sterilization(121 ℃,30min)
    Method Concentration 3~5 g/L

    This study conducted sterile inoculation of Vero cells on microcarriers in 2L and 10L glass vessels, controlling the process through a benchtop bioreactor. In this test, the microcarrier concentration was low, and regular culture methods were employed without the need for additional feeding or media changes, achieving cell proliferation and expansion. The cultured Vero cells attained a high density; with a microcarrier concentration of 3g/L and an initial seeding density of 1.2×105 cells/mL, by the fourth day, the cell density reached around 1.4×106 cells/mL, maintaining a viability of over 90%. Cells were harvested by enzymatically digesting them off the spheres and transferred to new or existing spherical carriers, enabling an expanded culture by transferring cells from one sphere to another. Additionally, increasing the carrier concentration can boost cell density. This confirms the significant potential of using LeProlif™ Sphere to cultivate Vero cells for vaccine production.

     

    Materials & Equipement

    In the 2L glass jar benchtop bioreactor, we conducted tests on the growth of Vero cells on spherical carriers to understand the proliferation characteristics of Vero cells within the benchtop bioreactor. Subsequently, we further amplified this process to the 10L glass bioreactor through bead-to-bead expansion, providing relevant references for the application of Vero cells in virus preparation.

    Conclusion

    With a carrier concentration of 3g/L and an initial cell seeding density of 1.2E5/mL, after 4 days of cultivation, scaling up from 1.5L to 4L through bead-to-bead expansion, and further scaling up to 10L, the cell density increased approximately 14-fold while maintaining a viability consistently above 90.0%. Across different scales of enlargement within glass jars, there was a high level of consistency observed, demonstrating favorable bead-to-bead effects, as depicted in Figures.

    Micro2

    Brochures

    • DownloadDownload
      Datasheet-LeProlif Sphere Microcarriers
    • DownloadDownload
      Manual-LeProlif Sphere Microcarrier

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